Quantitative extraction and determination of nonesterified fatty acids in plasma.

Colorimetric Determination of Free Fatty Acids in Biological Fluids.

Following the introduction by Duncombe of a colorimetric micromethod for the determination of free fatty acids (FFA) dissolved in chloroform, we studied the optimum conditions for chloroform extraction of FFA from blood or other biological fluids. A degree and rate of extraction similar to those with Dole’s extraction mixture were obtained. Phospholipids are not extracted by the procedure descr...

Determination of the fatty acid profile of neutral lipids, free fatty acids and phospholipids in human plasma.

BACKGROUND Knowledge of the fatty acid composition of lipid classes in human plasma is an important factor in the investigation of human metabolism. Therefore, a method for the analysis of neutral lipid (NL), phospholipid (PL) and free fatty acids (FFA) in human plasma has been developed and validated. METHODS Separation of lipid classes was carried out by solid phase extraction of the lipid ...

Quantitative and qualitative assessment of fatty acids of seeds in Armenian cucumber (Cucumis melo var. flexuosus)

Background & Aim:Armenian cucumber (Cucumis melo var. flexuosus) is one of the varieties of Cucurbitaceae family that is freshly consumed in Iran, while due to the presence of useful fatty acids and minerals, it can play a special role in the health diet. The aim of this study was to evaluate the quantitative and qualitative of fatty acids in the seeds of Armenian cucu...

Semi-automated micro-scale extraction apparatus for assay of free fatty acids in plasma.

We describe the use of a semi-automated micro-scale extraction apparatus as applied to the assay of free fatty acids in plasma. With it, a smaller plasma sample is required, disposable glassware can replace acid-washed glassware, and the number of samples that one technican can assay per day is increased fivefold. Theroretically, this apparatus can be applied to all assay procedures requiring o...

Automated enzymatic determination of plasma free fatty acids by centrifugal analysis